IL-6 anticorps (HRP)

N° du produit ABIN1043799

N° du produit (Fournisseur): 209-403-310

Cet anticorps anti-IL-6 est un anticorps Lapin Polyclonal détectant IL-6 dans WB, IHC et ELISA. Adapté pour Humain. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour IL-6 anticorps (HRP) (ABIN1043799)

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-6 est conjugé à/à la HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-IL-6 anticorps (HRP)

N° du produit (Fournisseur): 209-403-310

Fournisseur: Rockland

Fonction: IL-6 Antibody Peroxidase Conjugated

Réactivité croisée (Details): In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human Il-6 in cell supernatants and certain body fluids.

Attributs du produit: Synonyms: rabbit Anti-IL-6 peroxidase conjugated antibody, rabbit anti-interleukin-6 peroxidase conjugated antibody, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF

Purification: This purified antibody has been heated to 56°C for 30 minutes.

Immunogène:

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-6 produced in E.coli.

Immunogen Type: Recombinant Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500-1:2,500

Application Note: This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/μl by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:1,000-1:5,000

ELISA Dilution: 1:10,000-1:50,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.1 mg/ml

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-IL-6 (HRP) (ABIN1043799)

Novick: "Current concepts in the management of renovascular hypertension and ischemic renal failure." dans: American journal of kidney diseases : the official journal of the National Kidney Foundation, Vol. 13, Issue 6 Suppl 1, pp. 33-7, (1989) (PubMed).

Détails sur IL-6

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Autre désignation: IL6

Sujet: Background: IL-6 is a secreted cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.

ID gène: 3569

NCBI Accession: NP_000591

UniProt: P05231

Pathways: Signalisation TLR, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome